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ISO2
Sigma
Mouse Monoclonal Antibody Isotyping Reagents
sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD)
| Synonym: | Mouse Monoclonal Antibody Isotyping Reagents |
Description
| Application | Determination of the subclass of a monoclonal antibody is helpful for characterization of the antibody, for choosing detection reagents, and for deciding on a purification scheme. |
| Features and Benefits | • May be used in a variety of assay formats • Suitable for all antibody forms • Determines all mouse IgG subclasses, IgA, and IgM |
| Specificity | ISO-2 contains 0.2 mL vials of subclass specific antibodies to all four mouse IgG subclasses, IgA, and IgM. Directions are included for using these reagents in ELISA and Ouchterlony assays to determine the subclass of monoclonal antibodies in ascites, culture supernatants, or purified preparations. Additional reagents may be required, such as conjugates for ELISA assays or agarose and buffer components for Ouchterlony assays. |
Properties
| usage | sufficient for 1000 tests (clones) (by ELISA) |
| sufficient for 40 tests (clones) (by immunodiffusion, ODD) | |
| shipped in | wet ice |
| storage temp. | 2-8°C |
Components
| Standard component | directions for use with each application |
| goat antisera to mouse IgA 0.2 mL | |
| goat antisera to mouse IgG3 0.2 mL | |
| goat antisera to mouse IgG1 0.2 mL | |
| goat antisera to mouse IgG2a 0.2 mL | |
| goat antisera to mouse IgG2b 0.2 mL | |
| goat antisera to mouse IgM 0.2 mL |
